Items on display will include the actual shirt worn by centre forward, and scorer of the winning goal Bert Freeman – the first time it has been on show in public.
The club already owns, or has on long-term loan, a number of items, souvenir publications, documents and photographs relating to the cup final success at the Crystal Palace ground on April 25, 1914.
Clarets co-chairman Mike Garlick recently bought Mr Freeman’s famous shirt at an auction, and it will form the centrepiece of the exhibition, curated by club historian Ray Simpson, in Towneley’s Long Gallery.
And, in an unprecedented move, admission to the display will be free for all Turf Moor season ticket holders, residents of Burnley borough, students and under-16s.
Alongside the exhibition, fans will also get the first glimpse of the replica shirt which Sean Dyche’s side will wear in their anniversary fixture – and final home game of the Championship season – against Ipswich Town on April 26.
Fans will be hoping Burnely can gain promotion back to the top flight which would make the 100 years celebrations even more poignant.
Instead of the usual club crest, the shirt will bear a Royal Coat of Arms badge, as worn in 1914, in honour of the presence of His Majesty, King George V. The Final was the first watched by a reigning monarch.
The club had to obtain special permission from Buckingham Palace for the shirts to carry this crest.
The 1914 exhibition will be unveiled at a special invite-only event on April 6 and open to the public on April 7.
General admission for adults and senior citizens who do not live in Burnley or do not have Burnley season tickets will be £4.