The most notable of the new faces are Liverpool pair Jordon Ibe and Joe Gomez, who have both featured in all three Premier League matches for the club so far. Spurs midfielder Dele Alli is also selected after scoring his first Premier League goal at the weekend.
Calum Chambers, Eric Dier and Matt Targett have all been capped at this level in defence, with Wolverhampton Wanderers duo Kortney Hause and Dominic Iorfa making the step-up from last season’s U20s.
The likes of James Ward-Prowse, Nathaniel Chalobah, Ruben-Loftus-Cheek and Jake Forster-Caskey have all featured for Southgate in midfield previously.
Goalkeepers: Angus Gunn (Manchester City), Jordan Pickford (Preston North End, on loan from Sunderland), Christian Walton (Bury, on loan from Brighton & Hove Albion)
Defenders: Calum Chambers (Arsenal), Eric Dier (Tottenham Hotspur), Brendan Galloway (Everton), Joe Gomez (Liverpool), Kortney Hause (Wolverhampton Wanderers), Dominic Iorfa (Wolverhampton Wanderers), Jack Stephens (Middlesbrough, on loan from Southampton), Matt Targett (Southampton)
Midfielders: Dele Alli (Tottenham Hotspur), Nathaniel Chalobah (Chelsea), Jake Forster-Caskey (Brighton & Hove Albion), Ruben Loftus-Cheek (Chelsea), James Ward-Prowse (Southampton)
Forwards: Chuba Akpom (Hull City, on loan from Arsenal), Jordon Ibe (Liverpool), Solly March (Brighton & Hove Albion), Nathan Redmond (Norwich City), Duncan Watmore (Sunderland), James Wilson (Manchester United), Cauley Woodrow (Fulham)