The call comes amid mounting concern among fans over the future of the club, including growing anger on social media over the way Rovers is being run, a poor start to the season and falling attendances.
The call for the Ewood Park club to be put on the market also comes as the fifth anniversary of the trust’s initial request to meet and talk to its owners approaches.
It is the first time the supporters trust has formally called for a change of ownership at Rovers and comes as it says the relationship between fans and owners is deteriorating even further.
Members of the Trust were asked if they approved this new stance and 97% of those who responded said yes.
The organisation also says it is willing to work with other groups and explore all options to help see positive change take place for the good of the club.
Wayne Wild, chairman of the Rovers Trust, said: “We are calling on the owners to put Blackburn Rovers up for sale in a professional and public way that will encourage people to think about possible investment in the club.
“We know there are people out there who are definitely interested and the time is right".
Rovers had their lowest crowd for over 30 years when only 3,448 came through the turnstile and over 500 of those were from Crewe.
These are worrying times for Rovers fans.